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Though not required, it is better that you have an account on GitHub. This is used by Homestead a special vagrant box made for running Laravel apps. At the very top of the chain is Mix , which is basically an API for Webpack , which in turn requires npm which is basically a package manager for NodeJS.
Other optional stack components are Memcached , Redis and Beanstalkd. While you are not required to understand them all at once, it is advantagous that you are at least familiar with these and do some reading about what they are and where they are being used.
This will save you tons of confusion when reading the documentation and references in the future.
Patterns Principles and Practices of Domain Driven Design
Use the Current Stable Version 5. Laravel has added powerful new features over the last few years.
If you are looking for a function or its usage, the documentation on the laravel. You can use Homestead to start developing with Laravel without the hassle of setting up your local environment for Laravel.
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The instructions and usage of homestead can be found in the Official Documentation. Build your own Homestead Homestead comes with pre-installed software.
If you want to customize the installed software, you will need to have access to the scripts that is used to build Homestead itself. You can check the source code of that project to have a better understanding of how Homestead was built, and probably add your own scripts as well.
It is a good idea to get some background of these concepts first before diving into using the framework, so you will be able to make sense why the framework is designed this way. Service Provider Generally, a Service is a well-known set of programming interfaces and classes that provide access to some specific application functionality or feature. While a Service Provider Interface is an interface or abstract class that acts as a proxy or an endpoint to the service.
The Service Provider contains one or more concrete classes that implement or extends the service type. Dependency Injection is a technique whereby one object or static method supplies the dependencies of another object. A dependency is an object that can be used a service. Each circle represents one subset identified by the EPP approach. The size of the circle directly correlates with the relative frequency of the subset in the sample. X and Y axes are MDS coordinates.
This branching diagram starts by placing clusters with the smallest pairwise dissimilarity scores in the lowest branches of diagram; these pairs of clusters are further progressively merged in the next branching level of the QF-tree and further considered as one cluster; dissimilarity scores are then recalculated for all of the clusters on this branching level and the merging process is repeated. This process is sequentially repeated until all of the clusters identified within the sample are merged together.
We named this tree-structure data display as QF-tree Full size image To visualize clustering outcomes in a composite figure, we developed two data display alternatives that can supplement each other.
We use a multidimensional scaling MDS method 12 that allows placement of each object cluster in two-dimensional space such that the overall between-object distances in high-dimensional space are well-preserved. To make the results more visually interpretable, we apply this MDS method to the matrix of distances between median values calculated for each of the identified clusters Fig.
This reduces the effect of the crowding problem 13 and, importantly, allows computationally efficient application of MDS.
We also created a tree-structure data display Fig. This data display method builds the hierarchy from the individual clusters identified within one sample by progressively merging clusters.
In order to decide which clusters should be merged, a measure of dissimilarity between sets of observations is required.
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We named this tree-structure data display QF-tree. But as we previously demonstrated 6 , distance metrics such as quadratic form that take into account changes in both location and frequency rather than just changes in one or the other are the most suitable and accurate methods for comparing multivariate non-parametric flow-cytometry data distributions.
Here, we add Euclidean distance to quadratic form distance measure to ensure linear monotonic behavior for this dissimilarity measure see Fig. We refer to the above computational pipeline i. The algorithms constituting this pipeline are available as parts of the AutoGate software, which is freely available for download by not-for-profit users.
The python implementation provides alternative choices for MDS data display, including the use of median values or adaptive bins that are calculated for each of the identified clusters.
We show that the standard cell subset measurements i. Each circle corresponds to one identified cell subset, and the size of the circle represents relative cell frequiency. Matched cell subsets are labeled with the same color.
The length of edges corresponds to quadratic form score value Full size image Such strong agreement in median fluorescence values and cell frequencies between cell populations identified automatically by the SIC pipeline and those identified with user-guided clustering implies their substantial overlap in the space of measured parameters. However, to verify this point, for every cell population identified with the SIC pipeline, we performed backgating analysis to detect the location of the identified cell population within the gating tree built according to a conventional gating strategy.
In addition to identifying well-established immune cells subsets, the SIC pipeline was able to identify other cell subsets that were not considered within the established manual gating strategy. For example, using the same set of parameters as in the manual gating strategy, the SIC pipeline identified two subsets of dendritic cells DC based on the expression levels of surface CD11b Supplementary Fig.
In other words, QF-tree organized cells in a hierarchy of related phenotypes.
Although QF-tree can reliably reproduce patterns of hematopoiesis from high-dimensional cytometry data, its utility is limited by the choice of markers that are measured in the experiment. For instance, if the tree structure is built with a marker set that is not related to cellular progression, one might not expect to recover the known lineage relationships. We used the manual gating strategy shown in Supplementary Fig.
We further used the QFMatch algorithm to align these subsets with the subsets identified by fully automated EPP clustering. The QFMatch algorithm successfully aligned the immune cell subsets that were identified in a user-guided manner with those that were identified by a fully automated EPP.
The SIC pipeline consistently detected all the cell subsets that were identified by the manual gating strategy Fig. Unmatched cell subsets are indicated as red squares. These unmatched subsets are cell populations that were not identified by the user in the manual gating strategy.
In contrast, EPP is an exhaustive subset identification technique, i. These unmatched subsets are cell subsets that were not identified by the user in the conventional gating strategy, but they can now be readily explored looking at: a the expression level in each channel via pathfinder tool see Supplementary Fig.
This toolkit a—c was designed to interpret the fully automated clustering outcomes and assign cell subset names to identified clusters.
Also, this toolkit can help reveal the presence of a false cluster created by the EPP approach. However, subsequent studies have shown that this population is still heterogeneous and is, at best, enriched for HSCs.I had a lot of questions back in those days on the practicalities of the topics that Eric Evans explains in his book.
When connections must be made between different contexts, they tend to bleed into each other. The size of the circle directly correlates with the relative frequency of the subset in the sample. We used the manual gating strategy shown in Supplementary Fig.
We further used the QFMatch algorithm to align these subsets with the subsets identified by fully automated EPP clustering. Domain Event A domain object that defines an event something that happens. Inversion Of Control IoC inverts the flow of control as compared to traditional control flow.
Disadvantages[ edit ] In order to help maintain the model as a pure and helpful language construct, the team must typically implement a great deal of isolation and encapsulation within the domain model. In this context, a seat is actually a value object. But it was definitely worth turning over all pages.